분자생물학과 생물정보학의 기본적이고 핵심적인 개념을 마스터하세요.
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Getting Started
VIDEO SERIES
A Brief Tour of Geneious Prime
Get up and running quickly with this brief tour of the Geneious Prime interface.
VIDEO SERIES
Getting Started with Geneious Prime
Watch this series to get started with your molecular biology and sequence analysis in Geneious Prime.
GUIDE
Tips and Tricks
Discover tips and tricks for making the most of the tools, functions, shortcuts and features in Geneious Prime.
Next Generation Sequencing
VIDEO SERIES
De Novo Assembly
Watch the series and learn how to perform a de novo assembly of short-read NGS data and assemble circular contigs.
VIDEO SERIES
Map to Reference
Use the map to reference tool to map next-generation sequencing data against a reference, and assemble coronavirus genomes.
VIDEO SERIES
Call and Compare SNPs
Perform a reference assembly with next-generation sequencing (NGS) data and call SNPs on the assembled contig.
VIDEO SERIES
Metagenomics Analysis
In this in-depth video series learn how to assemble, filter and analyze an NGS amplicon metagenomic data set.
VIDEO SERIES
Expression Analysis
Calculate and compare normalized expression measures from RNA-Seq data using the Geneious expression analysis tool.
TUTORIAL
Practice De Novo Assembly
Use this practical exercise to perform a de novo assembly of short-read NGS data and assemble circular contigs.
TUTORIAL
Mapping and SNP Calling
With this practical exercise, perform a reference assembly with NGS data and call SNPs on the assembled contig.
TUTORIAL
Amplicon Metagenomics
Assemble, filter and analyze an NGS amplicon metagenomic data set in Geneious Prime with this practical exercise.
TUTORIAL
Practice Expression Analysis
Practice calculating normalized expression measures from RNA-Seq data using the Geneious expression analysis tool.
TUTORIAL
Expression Analysis with DESeq2
Use DESeq2 to compare expression levels for two sample conditions with replicates, in this practical exercise.
TUTORIAL
Sequence Classifier
Practice using the Sequence Classifier by classifying mitochondrial sequences obtained from subfossil bones.
Sanger Sequencing and Microsatellites
VIDEO SERIES
Sanger Sequence Analysis
Learn to align your chromatograms to a reference sequence, find variants and verify sequences in this video series.
VIDEO SERIES
Microsatellite Analysis
Use the Microsatellites Plugin to import ABI fragment analysis files, visualize traces, fit ladders, call peaks and more.
TUTORIAL
Assemble Chromatograms and Edit Sequences in Geneious Prime
Learn how to assemble chromatograms, edit bidirectional DNA sequences, and extract high-quality consensus sequences.
TUTORIAL
Analyze Microsatellite Traces
Practice using the Microsatellite plugin to fit a ladder, call peaks, bin alleles and produce a table of genotypes.
Alignments and Tree Building
VIDEO SERIES
Phylogenetic Trees
In this video learn the basic steps to building a phylogenetic tree and manipulate it using the Geneious tree viewer.
VIDEO SERIES
Multiple Alignments
Watch how to perform multiple alignments and assess levels of homology among sequences before constructing a phylogeny.
VIDEO SERIES
Pairwise Alignment
Use a dot plot to view regions of similarity then determine homology with pairwise alignment of related sequences.
VIDEO SERIES
Mauve Alignment
Watch the series and learn how to use the Mauve plugin. Align genomes and convert alignments into standard alignments.
TUTORIAL
Build Phylogenetic Trees
Practice aligning sequences, building a phylogenetic tree, and viewing and manipulating the tree.
TUTORIAL
Practice Pairwise Alignment
Use this practical exercise to align pairs of DNA and protein sequences using dot plots and alignment algorithms.
TUTORIAL
Alignment with Mauve
Practice using the Mauve plugin to align genomes, complete genomes, and order draft genomes against a reference.
TUTORIAL
Molecular Analysis of Plant DNA
Practice how to annotate plant DNA sequences using BLAST and multiple alignment. Read a phylogenetic tree.
Molecular Cloning
VIDEO SERIES
Restriction Cloning
Find and analyze restriction enzymes and simulate restriction cloning of a single insert into a vector backbone.
VIDEO SERIES
Gateway Cloning
Watch this video series to learn the basics of Gateway cloning and how to simulate single or multisite Gateway cloning.
VIDEO SERIES
Golden Gate Cloning
Watch the series to learn the basics of Golden Gate cloning and how to simulate standard or batch Golden Gate cloning.
VIDEO SERIES
Gibson Assembly
In this video learn to simulate Gibson Assembly using a single insert, and perform batch cloning from a sequence list.
VIDEO SERIES
In-Fusion Cloning
This video covers the basics of In-fusion cloning. Learn to simulate In-fusion cloning and analyze your results.
VIDEO SERIES
TOPO Cloning
Watch this 3 part series on simulating TOPO cloning in Geneious Prime using either the TA, blunt or directional method.
VIDEO SERIES
Parts Cloning
Learn how to simulate parts cloning and concatenate libraries of genetic elements outputting all possible combinations.
TUTORIAL
Practice Restriction Cloning
Practice simulating restriction cloning. Ligate PCR product into an expression vector and confirm CDS in-frame fusion.
TUTORIAL
Single Site Gateway Cloning
Practice simulating gateway cloning. Automatically design attB-linked primers, and the BP and LR reactions.
TUTORIAL
Multisite Gateway Cloning
Practice simulating multisite Gateway cloning. Choose attB sites, design primers, and simulate BP and LR reactions.
TUTORIAL
Practice Golden Gate Cloning
Practice simulating Golden Gate cloning. Automatically design primers to generate overhangs for parts assembly.
TUTORIAL
Practice Gibson Assembly
Practice simulating Gibson Assembly of a single insert, and perform batch cloning from a sequence list.
TUTORIAL
Practice TOPO Cloning
In this practical exercise, learn how to simulate TOPO cloning, using either TA, Blunt or Directional methods.
TUTORIAL
Perform Cloning Validation
Group reads into clones, map clones to a reference and automate validation of sequences with the cloning validation tool.
Primer Design and CRISPR
VIDEO SERIES
Primer Design
In this series learn how to view, design and export primers for many applications such as PCR, cloning or sequencing.
VIDEO SERIES
Working with CRISPR
In this series, learn to find, annotate and check CRISPR sites, prepare sequence files, analyze, and interpret results.
TUTORIAL
Practice Primer Design
Practice how to import, design and test oligonucleotide primers, primer-pairs and primer-pair/probe combinations.
TUTORIAL
Finding CRISPR Sites
Practice finding and annotating CRISPR sites in a sequence. Check against a genome for off-target binding sites.
TUTORIAL
Analyze CRISPR Editing
Practice analyzing CRISPR editing results, preparing sequences, setting up the analysis and interpreting results.
Annotate and Visualize
VIDEO SERIES
Annotate Sequences
Learn to annotate your sequences by customizing the view, adding new annotations or annotating your sequence with BLAST.
VIDEO SERIES
Viewing 3D Structures
This video covers the 3D structure viewer. Learn how to visualize and manipulate active sites in a protein structure.
TUTORIAL
Transfer Annotations by Homology
Use the Transfer Annotations tool to transfer features to a reference or consensus sequence in an alignment or assembly.
TUTORIAL
Transfer Annotations by Alignment
Practice transferring annotations between sequences within an alignment using the "Copy To..." function.
TUTORIAL
Transfer Annotations From Database
Practice how to transfer homologous annotations from a custom feature database using the "Annotate From" tool.
TUTORIAL
Visualize 3D Protein Structures
With this practical exercise, explore the controls in the 3D structure viewer. Visualize active sites in a protein structure.
BLAST, NCBI & GenBank
VIDEO SERIES
Searching with BLAST
Learn how to search with BLAST. Single or batch BLAST your sequence against GenBank or custom databases to find similar sequences.
VIDEO SERIES
Working with GenBank
Watch the series on how to access GenBank, and prepare and submit sequences and alignments to the GenBank database.
TUTORIAL
BLAST Searching
A practical exercise to BLAST your sequence against GenBank or custom databases to find similar sequences.
TUTORIAL
Accessing GenBank
Practice how to access information stored in the GenBank database through the Geneious interface.
TUTORIAL
Submitting to GenBank
Practice formatting sequences and alignments for submission to GenBank with the GenBank Submission tool.
Automation & Customization
VIDEO SERIES
Creating Automated Workflows
In this video series learn to use workflows to automate your analyses and run a set of operations with a single click.
TUTORIAL
Practice Building Automated Workflows
Practice setting up an automatic workflow to run a pipeline from Sanger sequences to a phylogenetic tree.
Learn Sequence Analysis
GUIDE
Introduction to DNA Sequencing
Learn the basics of DNA sequencing with this introductory article on Sanger, Next Generation and Long-read methods.
GUIDE
Assembling Your DNA Sequences
Learn the basics with this introductory guide to assembling DNA sequences with de novo and map to reference.
GUIDE
Understanding Sequence Alignment
Learn the basics of sequence alignment with this overview on the different methods used to align sequences.
GUIDE
Understanding Phylogenetics
Learn the basics of phylogenetics with this overview of phylogenetic treesm how they work and how to build them.
GUIDE
Introduction to Molecular Cloning Methods
Learn the basics in Molecular Cloning with this overview article that covers restriction enzyme cloning to golden gate cloning.