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Accessing Genbank

Once DNA has been sequenced it is deposited in a sequence database. The main databases which form the International Nucleotide Sequence Database Collaboration are GenBank, administered by the National Centre for Biotechnology Information (NCBI) in the USA (http://www.ncbi.nih.gov/Genbank), the European Molecular Biology Organisation (EMBO) database in Europe (http://www.ebi.ac.uk/), and the National DNA Databank of Japan (NDDBJ). These databases exchange information daily. For protein sequences the principal database is the SWISS-PROT database (http://us.expasy.org/sprot/). This also exchanges with GenBank, NDDBJ and EMBO.

Geneious Prime provides a user friendly interface into Genbank so that information is retrieved intuitively and visually, and can be easily integrated into your existing database.

In this tutorial, you will carry out a basic BLAST search of Genbank to identify a mystery sequence, and learn how to access information from a number of different Genbank databases

If you want to open the tutorial in a different window, you can click the new window button . If you wish to view this tutorial on the web, click here.


Exercise 1: Carrying out a BLAST search of the Genbank database
Exercise 2: Investigating the taxonomy of the organism
Exercise 3: Searching Genbank for further sequences from the same species
Exercise 4: Genome searching