Finding CRISPR Sites

In this tutorial you will use the CRISPR tool in Geneious Prime to search for "GN(20)GG" guide RNA (gRNA or "CRISPR") sites in the LYP1 gene from Saccharomyces cerevisiae (baker's yeast) and check for non-target binding sites against the rest of the Saccharomyces cerevisiae genome.


Introduction Introduction
Exercise 1: Finding CRISPR sites and checking for off-target matches
Exercise 2: Finding paired CRISPR sites

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